The web supplement to G. Mercier et al.
Nucleic Acids Research, 2005, Vol. 33, No. 20 p.6635-6643
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| A HAPLOID-SPECIFIC TRANSCRIPTIONAL RESPONSE TO IRRADIATION IN SACCHAROMYCES CEREVISIAE |
| G. MERCIER1, N. BERTHAULT1, N. TOULEIMAT1, F. KEPES2, G. FOUREL3, E. GILSON3 AND M. DUTREIX1# |
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1 CNRS-UMR 2027, Institut Curie, Bat. 110, Centre Universitaire, F-91405 Orsay, France 2 Programme d'Epigenomique, Bat. G3 93 rue Henri Rochefort, F- 91000 Evry, France 3 Laboratoire de Biologie Moleculaire de la Cellule, l'Ecole Normale Superieure de Lyon CNRS-ENS UMR5161, 46 allee d'Italie, F-69364 Lyon Cedex 07, France # correspondence should be addressed to Marie Dutreix |
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Eukaryotic cells respond to DNA damage by arresting the cell cycle and modulating gene expression to ensure efficient DNA repair. We used global transcriptome analysis to investigate the role of ploidy and mating-type in inducing the response to damage in various Saccharomyces cerevisiae strains. We observed a response to DNA damage specific to haploid strains that seemed to be controlled by chromatin regulatory proteins. Consistent with these microarray data, we found that mating-type factors controlled the chromatin-dependent silencing of a reporter gene. Both these analyses demonstrate the existence of an irradiation-specific response in strains (haploid or diploid) with only one mating-type factor. This response depends on the activities of Hdf1 and Sir2. Overall, our results suggest the existence of a new regulation pathway dependent on mating-type factors, chromatin structure remodeling, Sir2 and Hdf1 and independent of Mec1 kinase. |
| Download data: | |
| MIAME description | Mercier_2005_MIAME.doc (1Mb) |
| Microarray List | Mercier_2005_MicroarrayList.xls (18Kb) |
| Array Design description | Mercier_2005_ArrayDesign.zip (686Kb) |
| Raw Data | Mercier_2005_RawData.zip (36Mb) |
| Analysed Data | Mercier_2005_Ratios.xls (8Mb) |
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Web links: |
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| Publication | Get the publication from Nucleic Acids Research |
| UMR 2027 CNRS/IC, Recombination and genomic instability group | Visit their web site |
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