|SPECIFIC TRANSCRIPTIONAL RESPONSES INDUCED BY 8-METHOXYPSORALEN AND UVA IN YEAST|
|MICHèLE DARDALHON 1*, WAKA LIN 2,3, ALAIN NICOLAS 2, DIETRICH AVERBECK 1|
1 Institut Curie Section de Recherche, UMR2027 CNRS/I.C., LCR n°28 CEA, Centre Universitaire d’Orsay, Bât. 110, F- 91405 Orsay Cedex, France.
2 Institut Curie Section de Recherche, UMR 7147 CNRS,Université Pierre et Marie Curie, 26 rue d’Ulm, 75248 Paris Cedex 05, France.
3 Riken Discovery Institute, Genetic System Regulation Laboratory, Hirosawa 2-1, Wako, Saitama 351-0198, Japan
* correspondence should be addressed to M. Dardalhon
Treatment of eukaryotic cells with 8-methoxypsoralen plus UVA irradiation (8-MOP/UVA) induces pyrimidine monoadducts and interstrand crosslinks and initiates a cascade of events leading to cytotoxic, mutagenic and carcinogenic responses. Transcriptional activation plays an important part in these responses. Our previous study in Saccharomyces cerevisiae showed that the repair of these lesions involves the transient formation of DNA double-strand breaks and the enhanced expression of landmark DNA damage response genes such as RAD51, RNR2 and DUN1 as well as the Mec1/Rad53 kinase signaling cascade. We have now used DNA microarrays to examine genome-wide transcriptional changes produced after induction of 8-MOP/UVA photolesions. We found that 128 genes were strongly induced and 29 genes strongly repressed. Modifications in gene expression concern numerous biological processes. Compared to other genotoxic treatments, approximately 42% of the response genes were specific to 8-MOP/UVA treatment. In addition to common DNA damage response genes and genes induced by environmental stresses, a large fraction of 8-MOP/UVA response genes corresponds to membrane-related functions.
|Protocole description||Dardalhon_Lin_2006_protocole.doc (37Kb)|
|Microarray List||Dardalhon_Lin_2006_MicroarrayList.xls (12Kb)|
|Array Design description||Dardalhon_Lin_2006_ArrayDesign.xls (810Kb)|
|Analysed Data||Dardalhon_Lin_2006_Ratios.xls (3Mb)|
|Publication||Get the publication from FEMS yeast Res (2007) DOI: 10.1111/j.1567-1364.2007.00270.x|
|UMR 2027 CNRS/IC, Genotoxic damage and gene induction in eukaryotic cells||Visit their web site|
|Last modified July 11 2007 09:51:27|